Journal: PLoS ONE

Article Title: Differential Effects of Serum Heat Treatment on Chemotaxis and Phagocytosis by Human Neutrophils

doi: 10.1371/journal.pone.0054735

Figure Lengend Snippet: ( A ) Results of staining with FITC-conjugated anti-C3b. ( B ) Results of staining with Alexa-Fluor®-488-conjugated anti-IgG. In all cases, the particles had been incubated in three different buffers, i.e., serum-free HBSS (“plain”), HBSS with 10% untreated autologous serum (37°C-ASPO), and HBSS with 10% autologous serum that had been heat-treated at 56°C (56°C-ASPO). The legend included in (A) applies to all panels.

Article Snippet: Opsonized and plain zymosan particles were incubated with 1 µg/mL FITC-conjugated mouse anti-human C3b (Accurate Chemical & Scientific Corp., Westbury, NY, USA) or Alexa-Fluor®-488-conjugated goat anti-human IgG (Invitrogen) monoclonal antibodies at room temperature on a rotator for 30 min, and washed twice.

Techniques: Staining, Incubation

Journal: PLoS ONE

Article Title: Differential Effects of Serum Heat Treatment on Chemotaxis and Phagocytosis by Human Neutrophils

doi: 10.1371/journal.pone.0054735

Figure Lengend Snippet: ( A ) Results of staining with FITC-conjugated anti-C3b. ( B ) Results of staining with Alexa-Fluor®-488-conjugated anti-IgG. The first three columns depict example data obtained with sera from different, healthy donors. The rightmost column summarizes the results for a total of 3 donors. Values are block-centered means ± SD.

Article Snippet: Opsonized and plain zymosan particles were incubated with 1 µg/mL FITC-conjugated mouse anti-human C3b (Accurate Chemical & Scientific Corp., Westbury, NY, USA) or Alexa-Fluor®-488-conjugated goat anti-human IgG (Invitrogen) monoclonal antibodies at room temperature on a rotator for 30 min, and washed twice.

Techniques: Staining, Blocking Assay